• Users Online: 254
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Year : 2017  |  Volume : 42  |  Issue : 2  |  Page : 45-51

Development of ex-vivo expanded megakaryocyte progenitors for platelet recovery

1 Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Suez Canal, Egypt
2 Department of Physiology, Faculty of Medicine, Suez Canal University, Suez Canal, Egypt

Correspondence Address:
Hassan M Amany
Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Suez Canal
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ejh.ejh_42_16

Rights and Permissions

Context Megakaryocytes (MKs) can be produced using umbilical cord mononuclear cells and CD34 cells in the same cocktail media. Aim The aim of this study was to produce human MKs and platelets through ex-vivo culturing of human cord blood. Patients and methods Cord blood samples were collected under sterile conditions, separated using Ficoll–Hypaque technique for mononuclear separation, followed by magnetic separation for CD34+ cells; both types of cells were cultured in the same cocktail media and followed up for 14 days. This prospective ex-vivo study was conducted to determine the differentiation potential of haematopoietic stem cells isolated from cord blood towards megakeryopoiesis lineage. Cord blood was collected in the obstetric emergency room of Suez Canal University Hospital. Stem cell separation and induction of megakeryopoiesis were carried out in the Tissue Culture Unit, Physiology Department, Faculty of Medicine, Suez Canal University. Results Both types of cells showed success towards megakaryopoiesis; however, mononuclear cells showed better results compared with CD34 cells. Conclusion Umbilical cord stem cell culture could be a new easy method for ex-vivo MK progenitor production and final platelet recovery. This success would encourage further studies in ex-vivo megakeryopoiesis and in using it in different medical aspects.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded164    
    Comments [Add]    

Recommend this journal