Year : 2014 | Volume
: 39 | Issue : 4 | Page : 246--252
Murine double minute-2 expression in B-cell chronic lymphocytic leukemia: correlation with apoptosis and disease outcome
Ashraf M El-Hefni1, Tarek A El-Gohary2, Naglaa A Khalifa2
1 Hematology & Oncology Unit, Department of Internal Medicine, Zagazig University, Egypt
2 Department of Clinical Pathology, Faculty of Medicine, Zagazig University, Egypt
Introduction B-cell chronic lymphocytic leukemia (B-CLL) is characterized by accumulation of quiescent monoclonal CD5+ B cells, which arise from undefined defects in apoptotic cell death. murine double minute-2 (MDM2) oncoprotein exists in an autoregulatory feedback loop with the tumor suppressor protein p53 and block p53-mediated transactivation and apoptosis.
Aim of this work The aim of the study was to evaluate the overexpression of MDM2 in B-CLL patients and correlate it with apoptosis using annexin-V expression and disease outcome.
Patients and methods This study was conducted on 55 patients including 45 patients with typical B-CLL; their ages ranged from 55 to 72 years, with a mean age of 58.55 ± 3.9 years. Ten age-matched and sex-matched apparently healthy individuals were included as the control group. The diagnosis of B-CLL was based on clinical, hematological, immunophenotypic, and cytogenetic criteria. Annexin-V and MDM2 oncoprotein expression was assessed by flow cytometric analysis.
Results Our study revealed that MDM2 was overexpressed in 53% of B-CLL cases and it was significantly elevated in comparison with the control group (P < 0.001). There was an inverse correlation between MDM2 overexpression and apoptosis (P < 0.001). The mean level of MDM2 expression was significantly reduced after therapy compared with the pretreatment level in the responder group (P < 0.001), whereas there was no significant difference (P>0.05) in the nonresponder group. The expression of MDM2 significantly correlated with the percentage of CD38 expression, LDH level, and B2M level (P < 0.001, P < 0.001, and P < 0.001, respectively) and patients who had responded to treatment showed a significant increase in the level of annexin-V (P < 0.001). MDM2 expression, percentage of CD38 expression, and LDH and B2M levels were significantly higher in the high-risk patients compared with low-risk and intermediate-risk patients (P < 0.001, P < 0.01, P < 0.05, and P < 0.001, respectively).
Conclusion MDM2 expression was inversely correlated with apoptosis, with decreased MDM2 expression in responder patients.
Ashraf M El-Hefni
Hematology & Oncology Unit, Department of Internal Medicine, Faculty of Medicine, Zagazig University, Zagazig 44519
|How to cite this article:|
El-Hefni AM, El-Gohary TA, Khalifa NA. Murine double minute-2 expression in B-cell chronic lymphocytic leukemia: correlation with apoptosis and disease outcome.Egypt J Haematol 2014;39:246-252
|How to cite this URL:|
El-Hefni AM, El-Gohary TA, Khalifa NA. Murine double minute-2 expression in B-cell chronic lymphocytic leukemia: correlation with apoptosis and disease outcome. Egypt J Haematol [serial online] 2014 [cited 2022 May 19 ];39:246-252
Available from: http://www.ehj.eg.net/article.asp?issn=1110-1067;year=2014;volume=39;issue=4;spage=246;epage=252;aulast=El-Hefni;type=0