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  Indian J Med Microbiol
 

Figure 1 Agarose gel electrophoresis shows (a) amplification product (b) RSaI restriction digestion of PCR product. (a) PCR amplification; Lane 1: DNA ladder Lanes 2-8: amplification bands of 322bp. (b) products of DNA restriction, Lane 1: DNA ladder, Lanes 3 and 7: Ala789 homozygous, Lanes 2 and 4: Thr789 homozygous, Lanes 5 and 6: Thr789Ala heterozygous and Lane 8: negative control.

Figure 1 Agarose gel electrophoresis shows (a) amplification product (b) RSaI restriction digestion of PCR product. (a) PCR amplification; Lane 1: DNA ladder Lanes 2-8: amplification bands of 322bp. (b) products of DNA restriction, Lane 1: DNA ladder, Lanes 3 and 7: Ala789 homozygous, Lanes 2 and 4: Thr789 homozygous, Lanes 5 and 6: Thr789Ala heterozygous and Lane 8: negative control.