Figure 3 CD47 surface expression in patients with chronic lymphocytic leukaemia. 4×105 cells were stained with PE-CD47 and FITC-CD19 mAbs. The studied populations were defined as R1, R2 and R3 (‘Patients and methods’ section). (a) B-lymphocytes (R1) expressing CD47+CD19+ as dot plot in the upper right quadrant, (b) neutrophils (R2) expressing CD47+CD19− in the upper left quadrant, (c) monocytes (R3) with CD47+CD19− in the upper left quadrant, events were captured and analyzed by flow cytometry for subgroup IA; newly diagnosed, IB; treated patients, control participants. Dot plot is representative to one of the studied cases. (d) Histograms represent cells percentage (right) and CD47 mean fluorescence intensity (left) as mean±SD. *Statistical significance difference.