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  Indian J Med Microbiol
 

Figure 1 (A) Photomicrograph shows morphology of sickle red blood cells (SS-RBCs) in a positive control slide. Mag.X400. The micrograph illustrates numerous abnormal shape of red cells (white arrows) after treatment with a reducing agent (2% Na2S2O4) in normal saline at the end of 120 mins. The blue arrows point toward classical biconcave disks of normal red cells. (B) Photomicrograph shows effect of MPF-extract on SS-RBC morphology. Mag.X400. It shows how pretreatment of SS-RBCs with MPF/normal saline dilution inhibited sickling of RBCs on exposure to low oxygen tension milieu (after induction with 2% Na2S2O4).The micrograph reveals more of classical biconcave disks of normal red cells and cells with central parlor (blue arrows) as against the abnormal sickle cells (white arrows).

Figure 1 (A) Photomicrograph shows morphology of sickle red blood cells (SS-RBCs) in a positive control slide. <b>Mag</b>.X400. The micrograph illustrates numerous abnormal shape of red cells (<b>white arrows</b>) after treatment with a reducing agent (2% Na<sub>2</sub>S<sub>2</sub>O<sub>4</sub>) in normal saline at the end of 120 mins. The blue arrows point toward classical biconcave disks of normal red cells. (B) Photomicrograph shows effect of MPF-extract on SS-RBC morphology. <b>Mag</b>.X400. It shows how pretreatment of SS-RBCs with MPF/normal saline dilution inhibited sickling of RBCs on exposure to low oxygen tension milieu (after induction with 2% Na<sub>2</sub>S<sub>2</sub>O<sub>4</sub>).The micrograph reveals more of classical biconcave disks of normal red cells and cells with central parlor (<b>blue arrows</b>) as against the abnormal sickle cells (<b>white arrows</b>).